A Renowned Institute of Biochemistry and Physiology of Microorganisms in Russia has developed new Technologies in Biopreparation for Plant Protection and Bioremediation.

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Pseudobacterin-2 is a biofungicide of protective and stimulating effect, based on cells of the bacterium Pseudomonas aureofaciens. The preparation comprises live bacterial cells, which colonize roots, stems and leaves of plants and suppress the growth and development of phytopathogens. The protective effect results from the ability of bacterial cells to produce extracellular metabolites (phenazines, siderophores, etc.) suppressing the growth of phytopathogenic fungi and bacteria and inducing the own immunity of plants. . Besides, bacterial

cells synthesize indole-3-acetic acid (phytohormone) and organic acids, which convert insoluble phosphates of soil into the forms available for plants. Biofungicide Pseudobacterin-2 has demonstrated high biological efficiency (65-96%) for the following diseases: Cercosporella, helminthosporiosis and fusariose root rots, powdery mildew, helminthosporiosis, Septoriosis,

sclerotiniose, brown and stem rust of cereals, brown spot of cucumber leaves and fruit, brown spot of tomatoes, and fusariose, rhizoctoniose, Pythium root rots of protected-ground cucumbers and tomatoes

MICRO-ORGANISMS FOR PURIFICATION OF SOILS FROM

Experimental biopreparations of Nikaoil series for consumptionof light and  heavy oil fractions have been developed from selected associations of microorganisms – oil destructors on the basis of laboratory research data. Selected microorganisms are natural isolates, nontoxic and non-pathogenic. Microbial cultures actively utilize oil and oil products, including not readily

degradable, heavy oil fractions. Due to the ability of some microorganisms to degrade oil hydrocarbons at low temperatures, they can be used for bioremediation of polluted territories in cold climate. Microbial associations have been selected for stable functioning on oil products and utilization of different oil fractions. The efficiency of Nikaoil biopreparations intended to

consume light and heavy oil fractions has been demonstrated in field tests on the territories of Moscow, Tver, Kirov regions and West Siberia. During the field season, the biopreparations had destroyed kerosene and diesel fuel for 85-90%, oil for 55-60% and black oil for 25-45%, depending on experimental conditions

Compare: when mastitis is treated with antibiotics, milk can be used only on day 5-8 after the recovery.  Lysomast and lynomast will allow cattle-breeding farms to avoid great economic losses associated with mastitis in cows.

NOVEL PROGRESSIVE TECHNOLOGY  FOR SODIUM CITRATE AND

CITRIC ACID PRODUCTION

The technology is based on using non-potable ethyl alcohol including technical alcohol, ethanol-containing semiproducts, and alcohol industry waste as raw materials. The producer is a specially selected yeast strain yielding up to 100 g/l of citric acid or 135 g/l of sodium citrate. Fermentation proceeds in continuous and periodic duty. Citric acid and sodium citrate are isolated by novel original  technology directly from permeate without concentrated acids and alkalis. The  

processes of fermentation and isolation of citric acid and sodium citrate are carried out with standard microbioloigcal and chemical equipment. The novel technology reduces 1.5-fold the electric and thermal energy consumption per unit of the products and absolutely excludes gypsum accumulation, suggesting the process to be environmentally friendly.

Inexpensive substrate - low-quality ethanol (technical, alcohol-containing semiproducts, alcohol production waste);highly active and stable yeast strain yielding up to 100 g/l of citric acid; novel simplified method of product isolation, excluding several stages of conventional technology; low cost of the products; minimization of solid waste and decrease of discharge (higher environmental safety).

MICROBIAL  BIOSENSORS

Elements of biosensor construction used for the first time and offering

advantage over the known analogs:

q           specific strains of microorganisms Þ higher sensitivity and selectivity

        of analysis;

q           methods of receptor making (specific materials and immobilization       

        methods) Þ long-term functioning of analyzer;

q           removable receptor elements Þ lower labor consumption and lower

        cost of analysis;

q           wide range of analyzed compounds;

q           combination of homogeneous and heterogeneous methods of   

        assay.

Analytical instrumentation of new generation – biosensors – for detection of biologically active compounds of different nature. The attention is focused on biosensor models based on microbial cells, enzymes and immune components, development of new principles of analysis, industrial prototypes of biosensor analyzers for detection of carbons, alcohols, aromatic hydrocarbons, proteins, bacterial cells   

FERMENTATION COMPLEX KF-108

·         Simultaneous screening of up to eight strains-producers in the same conditions (temperature, pH, pO₂) or testing of one producer in different fermentation conditions;

·        Quick and efficient optimization of medium composition and particularly cultivation conditions;

·        Cultivation of bacteria, yeast, and mycelium fungi using different substrates, including insoluble plant waste (beet pulp, wheat bran, malt sprouts, etc);

·    Cultivation by nearly all known methods, including periodic, continuous and

      fed-batch cultivation.